<i>Jchain</i>-diphtheria toxin receptor mice allow for depletion of antibody-secreting cells and analysis of differentiation kinetics.

ABSTRACT: Antibody-secreting cells (ASCs) are generated following B cell activation and constitutively secrete antibodies. As such, ASCs play key roles in humoral immunity, including responses to pathogens, vaccination, and homeostatic clearance of cell debris. Therefore, understanding basic tenets of ASC biology such as their differentiation kinetics following B cell stimulation is of importance. We developed a mouse model which expresses simian heparin-binding EGF-like growth factor (HBEGF) (diphtheria toxin receptor [DTR]) under the control of the endogenous Jchain locus (J-DTR). ASCs from J-DTR mice expressed high levels of cell surface DTR and were acutely depleted following diphtheria toxin treatment. Furthermore, proof-of-principle experiments demonstrated the ability to

SUBMITTER: Pioli KT 

PROVIDER: S-EPMC12666722 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature