Genome-Wide Blood DNA Methylation Profiling in Birch Pollen Allergic Patients Undergoing Allergen-Specific Immunotherapy.

ABSTRACT:

Background

Until now, no study has investigated the impact of allergen immunotherapy (AIT) on genome-wide DNA methylation in a longitudinal set-up. Herein, we investigated whether differences in DNA methylation occur in birch pollen allergic patients undergoing 6 months of birch pollen AIT, assessed alterations in methylation-based blood cell type composition, and correlated DNA methylation to serological AIT biomarkers.

Methods

We performed genome-wide DNA-methylation analysis on bisulfite-converted DNA derived from whole blood samples of 16 birch pollen-allergic patients (pre-/post-birch pollen AIT) and 15 placebo (pre-/post-placebo treatment).

Results

Our analysis identified cg22187251, located within a regulatory region upstream of the glucosaminyl (N-acetyl) tran

SUBMITTER: Lahnsteiner A 

PROVIDER: S-EPMC12666740 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature