Inverse Design of DNA-Programmable Protein Colloidal Crystals.

ABSTRACT: Protein crystals are a promising class of porous materials with applications in catalysis, encapsulation, and structural biology. However, the chemical complexity of proteins renders their crystallization a largely empirical process with variable success. In contrast, DNA-functionalized nanoparticles have been crystallized into ordered superlattices with predictable architectures using highly specific and tunable DNA-DNA interactions. Such programmability, however, has not yet been realized in protein crystals. Herein, we chemically modify protein oligomers with a DNA shell and crystallize the resulting protein-DNA conjugates using the principles of colloidal crystal engineering with DNA. When a new type of DNA design comprising three spacer-18 units and a 4-6 nucleotide sticky end was use

SUBMITTER: Han Z 

PROVIDER: S-EPMC12666855 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature