The transcription factor PoMbp1 promotes the growth and development of <i>Pleurotus ostreatus</i> by regulating polysaccharide utilisation.

ABSTRACT: The transcription factor Mbp1 was reported to regulate cell growth and proliferation in some fungi; however, its role in Pleurotus ostreatus remains largely unexplored. Here, we cloned the PoMbp1 gene, which encodes a protein contains an APSES domain and is localised in the nucleus. The expression pattern analysis showed that PoMbp1 was expressed more in the caps of the fruiting body than in other stages. The growth rates of the PoMbp1 overexpression strains OE-PoMbp1 were similar to those of wild-type, while that in the interference strains RNAi-PoMbp1 were markedly reduced, indicating that PoMbp1 positively regulates P. ostreatus development. Transcriptome sequencing showed that the DEGs between PoMbp1 transformants and wild-

SUBMITTER: Zhang L 

PROVIDER: S-EPMC12667323 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature