Isoform-specific oxidative modifications of tropoelastin by HOCl and MPO alter protein self-assembly.

ABSTRACT:

Background

Tropoelastin (TE), the soluble precursor of elastin, is critical for the elasticity of arteries, lungs, and skin. Oxidative damage to TE has been implicated in vascular diseases, but the isoform-specific effects remain poorly understood. Hypochlorous acid (HOCl), generated by the enzyme myeloperoxidase (MPO) targets extracellular matrix proteins during inflammatory processes. However, the differential susceptibility and functional consequences in specific TE isoforms are unknown.

Methods

We investigated the effects of HOCl and MPO-derived oxidants on two human TE isoforms, TE2 and TE6. Oxidative modifications were analyzed using high-resolution LC-MS/MS, with site-specific identification of chlorinated tyrosines and oxidized cysteine residues. Functional consequen

SUBMITTER: Mikkelsen KL 

PROVIDER: S-EPMC12667354 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature