Spatial Transcriptomics Reveals Transcriptomic and Immune Microenvironment Reprogramming during Thyroid Carcinoma Dedifferentiation.

ABSTRACT: Anaplastic thyroid carcinoma (ATC) is one of the most lethal human malignancies, often evolving from differentiated thyroid carcinoma (DTC) through a poorly understood dedifferentiation process. To elucidate this transition, spatial transcriptomic sequencing (spRNAseq) is performed on seven samples containing coexisting regions of ATC, poorly differentiated thyroid carcinoma, and DTC. SpRNAseq revealed that ATC regions were characterized by upregulated genes involved in immune suppression, angiogenesis, and extracellular matrix remodeling. Whole-exome sequencing and inferCNV analysis confirmed that adjacent DTC regions harbored mutational burdens comparable to those of ATC regions, suggesting early genomic priming for dedifferentiation. Trajectory analysis delineated a stepwise reprogrammi

SUBMITTER: Ning K 

PROVIDER: S-EPMC12667454 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature