One-Pot Encapsulation of Enzymes in a Calcium Carboxylate Metal-Organic Framework for Improved Buffer Stability.

ABSTRACT: Diverse metal-organic frameworks (MOFs) have been actively studied for enzyme encapsulation to enhance encapsulation efficiency (EE), retained enzymatic activity (REA), and stability. This study develops a biocompatible synthesis protocol for a MOF constructed from Ca²⁺ and 4,5-imidazoledicarboxylate ligand (termed CaIDC) in water at room temperature. This method enabled in situ, one-pot encapsulation of various enzymes, including bovine serum albumin (BSA), glucose oxidase (GOx), catalase (CAT), and esterase (EST). Compared to widely studied zeolitic imidazolate frameworks (ZIFs), CaIDC-based biocomposites demonstrated enhanced chemical robustness in phosphate buffer at pH 6.0 and 7.4. To optimize the synthesis process, a machine learning-assisted workflow incorporating Latin h

SUBMITTER: Shu Y 

PROVIDER: S-EPMC12667492 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature