Engineered Fusion Enzyme-Mediated Non-Consecutive Cyclization-Glycosylation Enables Heterologous Synthesis of Antifungal Enfumafungin.

ABSTRACT: Enfumafungin-type antibiotics, represented by enfumafungin and fuscoatroside, constitute a distinct class of fungi-derived fernane-type triterpenoids renowned for their potent antifungal activity. Notably, ibrexafungerp, a semi-chemically synthesized analogue of enfumafungin, has recently received approval as a novel antifungal drug. Thus, reconstituting the heterologous biosynthesis of enfumafungin holds great significance, as it offers a promising route for high-level production. Herein, the Aspergillus oryzae S184 chassis is first optimized. By deleting ku80 gene and refining counter-selection procedure, site-specific gene integration and substantially shortened the time required for selection marker recycling are significantly enhanced. Subsequently, an artificial biosynthetic pathway

SUBMITTER: Gao Y 

PROVIDER: S-EPMC12667498 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature