Inhibition of Macrophage ARID3A Alleviates Myocardial Ischemia-Reperfusion Injury After Heart Transplantation by Reducing THBS1/CD47 Signaling-Mediated Neutrophil Extracellular Traps Formation.

ABSTRACT: Mitigating myocardial ischemia-reperfusion (IR) injury is essential for enhancing the success of heart transplantation (HT) and improving patient outcomes. During HT, infiltrating neutrophils are influenced and regulated by various other cell types, contributing to myocardial IR injury through the excessive release of neutrophil extracellular traps (NETs). Nonetheless, the precise mechanisms underlying the interactions between neutrophils and other non-cardiomyocytes remain largely unexplored. Single-cell RNA sequencing is employed to characterize the cellular landscape and to explore the crosstalk between neutrophils and other non-cardiomyocytes. The role of AT-rich interactive domain-containing protein 3A (ARID3A) during HT is further examined using myeloid-specific ARID3A-knockout mice.

SUBMITTER: Tian H 

PROVIDER: S-EPMC12667509 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature