RING-Between-RING-Type E3 Ligase Ariadne-Like Protein 8 Negatively Regulates Plant Virus Infection by Targeting a Viral Movement Protein.

ABSTRACT: The ubiquitin-proteasome system is a highly conserved machinery that plays a crucial role in plant defense against viruses. However, the number of E3 ligases targeting viral proteins remains limited. Although RING-between-RING (RBR)-type E3 ligases are evolutionarily conserved across organisms, their functions in plant responses to biotic stress remain largely unknown. Herein, it is found that the triple gene block 1 (TGB1) protein of the barley stripe mosaic virus (BSMV) undergoes ubiquitination during viral infection. Immunoprecipitation combined with mass spectrometry identified an RBR-type E3 ligase that interacted with TGB1 in vivo and in vitro. The overexpression of Ariadne-like protein 8 (ARI8) inhibits, whereas its knockout enhances, the local and systemic spread of BSMV. ARI8 medi

SUBMITTER: Li W 

PROVIDER: S-EPMC12667515 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature