Stage-Resolved Phosphoproteomic Landscape of Mouse Spermiogenesis Reveals Key Kinase Signaling in Sperm Morphogenesis.

ABSTRACT: Spermiogenesis is the committed step of sperm production, during which spermatid cells undergo dramatic morphological transformations and transcriptional silencing. Post-translational modifications (PTMs), including phosphorylation, provide a level of protein function flexibility and play important roles in spermiogenesis. Dynamic protein phosphorylation profiles of spermatids are characterized across four different developing steps, and identified phosphorylation regulation of key proteins in spermiogenesis. Expression module and kinase-substrate phosphorylation network analysis revealed significant kinase activities of CSNK1G1 and TTBK2. CSNK1G1 is localized in the acrosome and is indispensable for acrosome biogenesis. Ttbk2 male germ cell conditional knockout mice are infertile with fla

SUBMITTER: Zhu T 

PROVIDER: S-EPMC12667522 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature