Integrative Genomics Approach Identifies Glial Transcriptomic Dysregulation and Risk in the Cortex of Individuals With Alcohol Use Disorder.

ABSTRACT:

Background

Alcohol use disorder (AUD) is a prevalent neuropsychiatric disorder that is a major global health concern, affecting millions of people worldwide. Previous studies of AUD used underpowered single-cell analysis or bulk homogenates of postmortem brain tissue, which obscure gene expression changes in specific cell types. Therefore, we sought to conduct the largest-to-date single-nucleus RNA sequencing (snRNA-seq) postmortem brain study in AUD to elucidate transcriptomic pathology with cell type-specific resolution.

Methods

Here, we performed snRNA-seq and high-dimensional network analysis of 73 postmortem samples from individuals with AUD (n = 36, n_nuclei = 248,873) and neurotypical control individuals (n = 37, n_nuclei = 210,573) in the dorsolat

SUBMITTER: Warden AS 

PROVIDER: S-EPMC12667600 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature