Whole 16S rRNA gene sequencing reveals differences in the oral microbiota of healthy individuals infected with oral HPV.

ABSTRACT:

Aim

To explore the oral microbiota in healthy individuals with and without oral human papillomavirus (HPV) infection and identify any features or changes in the oral microbiota associated with intermediate HPV infection.

Materials and methods

PacBio HiFi sequencing of the whole 16S rRNA gene was performed on 128 saliva samples from a previously characterized cohort (64 HPV-positive and 64 HPV-negative). Statistical analysis of alpha- and beta-diversity, as well as taxonomic composition (differential abundance testing), were used to determine differences between-subject groups.

Results

We demonstrated (1) significant differences in the abundance of Streptococcus salivarius in oral HPV-negative males; (2) males with low-risk HPV had an increased abundance of seve

SUBMITTER: Maidment TI 

PROVIDER: S-EPMC12667644 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature