Microsecond Time-Resolved Cryo-EM Based on Jet Vitrification.

ABSTRACT: Understanding and ultimately predicting the function of proteins is one of the frontiers in structural biology. This will only be possible if it becomes feasible to routinely observe proteins on the fast timescales on which they perform their tasks. Recently, laser flash melting and revitrification experiments have improved the time resolution of cryo-electron microscopy (cryo-EM) to microseconds, rendering it fast enough to observe the domain motions of proteins that are frequently linked to function. However, observations have been limited to a time window of just a few hundred microseconds. Here, we introduce time-resolved cryo-EM experiments based on jet vitrification that combine microsecond resolution with an observation window of up to seconds. We use a short laser pulse to initiate

SUBMITTER: Haubner M 

PROVIDER: S-EPMC12667775 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature