Description of <i>Gopheromyces tardescens</i>, gen. nov., sp.nov., <i>Gigasporangiomyces pilosus</i>, gen. nov., sp.nov., <i>Kelyphomyces adhaerens</i>, gen. nov., sp. nov., proposal of <i>Testudinimycetales</i> ord. nov. and <i>Testudinimycetaceae</i> fam. nov., and emended description of the order <i>Neocallimastigales</i>.

ABSTRACT: Anaerobic gut fungi (AGF, Neocallimastigomycota) represent a phylum of zoospore-producing fungi inhabiting the gastrointestinal tracts of herbivores. Twenty mammalian-affiliated genera (M-AGF) and two tortoise-affiliated genera (T-AGF) have been described so far. Here, we report on three additional novel T-AGF isolates obtained from Texas and sulcata tortoises. Phylogenetic analysis using the D1-D2 regions of the large ribosomal RNA subunit (D1-D2 LSU), RNA polymerase II large subunit (RPB1), internal transcribed spacer-1 region (ITS1), and transcriptomics-enabled phylogenomic analysis clustered these strains into three distinct, deep-branching clades, closely related to previously described T-AGF genus Testudinimyces. All isolates displayed filamentous rhizoidal growth patte

SUBMITTER: Morris A 

PROVIDER: S-EPMC12667834 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature