EBV INFECTION OUTCOMES DETERMINED BY MONOCYTE AND T_REG-DRIVEN IMMUNE DYNAMICS IN AN EX VIVO PBMC MODEL.

ABSTRACT: Epstein-Barr virus (EBV) infects >95% of the adult population with diverse outcomes ranging from benign latency to cancers and autoimmune diseases. Immunological control of EBV infection is known to be an important determinant of EBV infection outcomes. However, species-specific viral tropism and limited infection models have impeded mechanistic insights into early host-immune control of EBV infection. Here, we use ex vivo infection of peripheral blood mononuclear cells (PBMCs), rather than routinely used B cell enriched culture systems, to study immune and viral dynamics during primary EBV infection. We combined bulk RNA sequencing, EBV transcript enrichment, and flow cytometry to characterize cellular responses across Days 1, 7-8, and 14 post-infection. Early infection triggered a

SUBMITTER: Yoon L 

PROVIDER: S-EPMC12667846 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature