Unconventional linkers facilitate potent stabilized coronavirus stem antibody responses following nanoparticle vaccination.

ABSTRACT: Vaccine technologies that protect against a range of related pathogens within viral families, such as human immunodeficiency virus (HIV), influenza, and coronaviruses (CoVs) represent the future of viral vaccine development. Towards developing broad-spectrum CoV and influenza vaccines, we and others previously designed and evaluated CoV and influenza stem antigens; but these elicited relatively weak and sub-neutralizing antibody (Ab) responses. Multivalent antigen display on nanoparticles (NPs) is an established strategy to enhance and shape immunogenicity. However, one facet of NP vaccines has been largely overlooked: the indispensable linker segment between the antigen and NP core. Here, we introduce de novo-designed rigid (L2) and rarely used long flexible (L6) linkers to optimal

SUBMITTER: Dzuvor CKO 

PROVIDER: S-EPMC12667850 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature