RNA switch model for localization and translation of the myelin basic protein mRNA.

ABSTRACT: Oligodendrocytes myelinate the central nervous system by extending cellular projections that ensheath axons and elongate to form lipid-rich myelin. Classic studies visualizing RNA dynamics showed that myelin basic protein (MBP), one of the most abundant myelin proteins, is locally synthesized at the myelin sheath through the transport and local translation of Mbp mRNA. Mbp transport requires its 1.5-kb 3' untranslated region (3' UTR) and prior work identified candidate sub-sequences that may act as cis-acting transport stimulating RNA elements, including one with putative secondary structure. Here, a high-throughput reporter assay, dimethyl sulfate (DMS)-based RNA structure probing , and microscopy in primary rat oligodendrocytes identify a structured 127-nt region tha

SUBMITTER: Topkar VV 

PROVIDER: S-EPMC12667860 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature