Spatial regulation of AMPK activity under oxidative stress requires LKB1.

ABSTRACT: AMP-activated protein kinase (AMPK) is a central regulator of cellular energy homeostasis, with over 100 identified downstream targets throughout the cell. In response to cellular stress, including energetic stress, AMPK is activated via binding of AMP and phosphorylation by upstream kinases, including liver kinase B1 (LKB1) and calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2). We and others have found that the activation of AMPK in response to cellular stress has distinct subcellular mechanisms, indicating compartmentalized regulation of AMPK signaling. Although oxidative stress is known to stimulate AMPK activity, how AMPK is spatially regulated by oxidative stress is underexplored. Using a single-fluorophore excitation-ratiometric AMPK activity reporter (ExRai AMPKAR), we f

SUBMITTER: Parks K 

PROVIDER: S-EPMC12667911 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature