Inactivation of microtubule organizing center function of the centrosome is required for neuronal development and centriole elimination.

ABSTRACT: Cell differentiation is marked by a dramatic reorganization of the microtubule cytoskeleton that enables diverse cell functions. Mitotic precursor cells arrange microtubules around centrosomes, which become activated as microtubule-organizing centers (MTOCs) through the recruitment of pericentriolar material (PCM) and microtubules to build the mitotic spindle. A hallmark of differentiation is the "inactivation" of centrosomal MTOC function through the loss of PCM and microtubules, yet the function of this inactivation is unknown. We developed a GFP-nanobody-based targeting tool to activate centrosomes in C. elegans differentiated cells. Ectopically activating centrosomes in sensory neurons perturbed microtubule polarity, dynein-mediated trafficking, and caused defects in cell morpho

SUBMITTER: Ng R 

PROVIDER: S-EPMC12667983 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature