Biosynthesis of peptidic thiooxazole metallophores installed by multinuclear nonheme iron enzymes.

ABSTRACT: Significant effort has been directed toward characterization of nonheme iron enzymes owing to their breadth of unique reactivity. Through genome mining, we identified a conserved biosynthetic gene cluster within Pseudomonadota encoding one such family, the multinuclear nonheme iron-dependent oxidative enzymes (MNIO, formerly DUF692). Using a representative gene cluster from Fontimonas thermophila, we heterologously produced the post-translationally modified peptide fontiphorin, and detailed spectral analysis revealed MNIO-catalyzed installation of seven 5-thiooxazole (5TO) moieties. During our work, additional MNIO products were reported with conflicting structural assignments, so we investigated the related biosynthetic gene clusters from Haemophilus influenzae and Neisse

SUBMITTER: Gadgil MG 

PROVIDER: S-EPMC12668002 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature