Spatial Multi-Omics Workflow and Analytical Guidelines for Alzheimer's Neuropathology.

ABSTRACT: Spatial biology technologies enable high-dimensional profiling within intact tissues, revealing how molecular and cellular organization drives function and disease. As these platforms gain broader adoption, standardized analytical frameworks are needed to ensure data quality and reproducibility. Here, we present an end-to-end pipeline for the GeoMx Digital Spatial Profiler that simultaneously generates whole-transcriptome and 637-protein measurements from user-defined regions within the same tissue sections. The workflow integrates morphology-guided region selection, quality control, normalization, and multi-modal data interpretation. Applied to formalin-fixed cortical tissues from Alzheimer's disease, dementia with Lewy bodies, amyotrophic lateral sclerosis, and controls, the framework re

SUBMITTER: Sun X 

PROVIDER: S-EPMC12668010 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature