Establishment, validation and application of a spectrophotometric method for the accurate determination of carbonic anhydrase activity.

ABSTRACT: Carbonic anhydrase (CA) has been proven to be a rather important enzyme in both medical research and industrial development, leading to great application value in accurately quantifying CA enzymatic activity. This study focuses on developing a novel spectrophotometric method for measuring the CA enzyme activity with enhanced precision, reliability, and an ultra-low limit of detection (LOD) through the transition of changing pH values into a stable UV absorbance signal by the colorimetric probe of bromothymol blue (BTB), thereby addressing the limitations of existing assay techniques. Through the establishment of a colorimetric reaction system, optimization of the CA assay reaction system, and data analysis by both least squares and two-point methods, CA conventional and trace standard curv

SUBMITTER: Liu X 

PROVIDER: S-EPMC12668187 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature