Enhanced detection of HBV and HCV using Cas13a-FLAP and FGoAI platforms.

ABSTRACT: Hepatitis viruses continue to pose a major global health burden, underscoring the critical role of early diagnosis in achieving effective disease control. Here, a ratiometric fluorescence biosensor based on Cas13a and fluorescent RNA aptamers was developed for the highly efficient detection of hepatitis viruses. The integrated system consists of three functionally coupled modules: (i) duplex-specific nuclease (DSN)-enabled target recognition and sequence-specific cleavage, (ii) Cas13a-activated collateral degradation, and (iii) fluorescent RNA aptamer-based ratiometric biosensor. The proof-of-concept evaluation established limits of detection of 7.4 copies per µL for the hepatitis B virus (HBV) gene and 2.9 copies per µL for the hepatitis C virus (HCV) gene, respectively. Subsequently, ima

SUBMITTER: Gu X 

PROVIDER: S-EPMC12668212 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature