Transcriptome-wide mRNP condensation precedes stress granule formation and excludes new mRNAs.

ABSTRACT: Stress-induced messenger ribonucleoprotein (mRNP) condensation is conserved across eukaryotes, resulting in stress granule formation under intense stresses, yet the mRNA composition and function of these condensates remain unclear. Exposure of ribosome-free mRNA following stress is thought to cause condensation and stress granule formation through mRNA-sequence-dependent interactions, leading to disproportionate condensation of long mRNAs. Here, we show that, by contrast, virtually all mRNAs condense in response to multiple stresses in budding yeast with minor length dependence and often without stress granule formation. New transcripts escape mRNP condensation, enabling their selective translation. Inhibiting translation initiation causes formation of mRNP condensates distinct from stress

SUBMITTER: Glauninger H 

PROVIDER: S-EPMC12668231 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature