Viral replication and interferon responses in bronchial epithelia is enhanced by Th17 cells.

ABSTRACT:

Rationale

The impact of Th17 lymphocytes on epithelial responses to rhinovirus infection in asthma is poorly characterized.

Methods

Bronchial epithelial cells (BECs) from children with asthma were differentiated to an organotypic epithelium and primed via co-culture with healthy donor Th17 lymphocytes for 4 days prior to apical infection with human rhinovirus-16 (RV-16). RNA sequencing with WGCNA analysis was performed to identify modules of gene expression altered by Th17 priming or RV-16 infection in BECs or Th17 cells. Gene expression was correlated with viral copy number and with secreted protein levels.

Results

Analysis identified 4,030 genes grouped into 9 named modules with differential gene expression in BECs due to Th17 priming and viral infection. Modules wi

SUBMITTER: Powell WT 

PROVIDER: S-EPMC12668410 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature