Acute endothelial stresses identify microRNA let-7b-5p and non-coding SLC11A2 (NRAMP2/DMT1) exon as biomarkers that overlap with those detected in malignant and non-malignant diseases.

ABSTRACT:

Background

Disease biomarkers are often identified long after initiating pathologies, hampering mechanistic understanding and the development of preventative strategies. We hypothesized that aberrant cellular responses to normally-encountered stresses may be relevant to later disease states.

Aim

To model two under-explored acute cellular stresses for blood-exposed cells, and cross-reference to known biomarkers of disease.

Methods

Normal primary human endothelial cells (ECs) were treated for 1-6 h with cycloheximide (CHX) 100 μg/ml to inhibit protein translation and nonsense-mediated decay (modelling the integrated stress response), or 10 μmol/l ferric citrate (modelling diurnal variation in serum iron that can be augmented by treatments prescribed 8 million times/year

SUBMITTER: Bielowka AM 

PROVIDER: S-EPMC12668437 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature