DNA N6-methyladenine modifications of Acidithiobacillus ferrooxidans response to copper stress.

ABSTRACT: High concentrations of copper ions have long been recognized as a key factor limiting the efficiency of bioleaching due to the metal toxicity to microorganisms. In order to identify new determinants of copper resistance, we assessed the impact of different copper ion concentrations on the bioleaching model organism Acidithiobacillus ferrooxidans. Furthermore, we employed 6mA IP-seq technique to evaluate changes in the 6mA methylation levels of A. ferrooxidans under two conditions: iron oxidation and sulfur oxidation, both under copper stress. The results indicated that as the concentration of copper ions in the growth environment increased, the copper toxicity significantly inhibited the growth of A. ferrooxidans. The maximum tolerable copper ion concentration for iron-grown and sulfur-gro

SUBMITTER: Liu J 

PROVIDER: S-EPMC12668529 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature