A reliable in vitro rumen culture system and workflow for screening anti-methanogenic compounds.

ABSTRACT: Arguably the biggest man-made challenge of the century is halting climate change. Livestock's methane (CH4) emissions, a greenhouse gas with a higher global warming potential than carbon dioxide (CO2), represent a prime target for reducing anthropogenic impact. While the reduction of enteric methane emissions through feed additives has been demonstrated, potent and affordable compounds inhibiting methanogenesis in ruminants are not yet well established. Reliable methods for reproducible cultivation of the rumen microbiome in the laboratory are an essential tool for the study of methanogenesis. We have developed a versatile setup that allows for the cultivation of the ruminal microbiome in a benchtop configuration and combines, miniaturizes, and improves existing systems. The design is base

SUBMITTER: Laric PP 

PROVIDER: S-EPMC12668615 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature