Harnessing 3D cultured MSC exosomes through tangential flow filtration for enhanced diabetic wound healing.

ABSTRACT:

Introduction

Mesenchymal stem cell-derived exosomes have garnered considerable attention in regenerative medicine due to their non-immunogenicity, low infusion toxicity, easy accessibility, straightforward preservation, and minimal ethical concerns. While ultracentrifugation is the prevailing method for high-purity exosome isolation, it is limited by low throughput and the need for specialized infrastructure. This study investigates tangential flow filtration (TFF) as a promising alternative for exosome isolation. This technique offers simpler operation, higher yields, and improved recovery rates compared to ultracentrifugation.

Methods

Human umbilical cord mesenchymal stem cells (hUCMSCs) were cultured in a 3D microcarrier-bioreactor system, and exosomes were extracted from

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12668682 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature