Glial cell line-derived neurotrophic factor inhibits mast-cell-like RBL-2H3 cells activation via Ca2+-mediated degranulation and Ca2+/CaMKⅡ/JNK pathway.

ABSTRACT:

Introduction

Mast cells are important component of the intestinal immune system, play a crucial role in the pathogenesis of inflammatory bowel disease. Glial cell line-derived neurotrophic factor (GDNF), as a multifunctional growth factor, has recently garnered attention for its role in the inhibition of mast cells activation. This study aims to explore the potential mechanisms by which GDNF inhibits mast cell activation.

Methods

In this study, RBL-2H3 cells were used as an in vitro cell model of mast cells, which were cultured and treated with various interventions prior to collection of cells and culture supernatants. IgE-mediated degranulation were evaluated through β-hexosaminidase release assays. Culture supernatants were analyzed for TNFα, IL-1β, and IL-6 secret

SUBMITTER: Huang W 

PROVIDER: S-EPMC12669010 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature