Energy-transfer photoproximity labelling in live cells using an organic cofactor.

ABSTRACT: Photocatalytic proximity labelling has emerged as a powerful tool to resolve a variety of biomolecular and cellular interactions. Although the use of high-resolution probes, such as diazirines, enables cell-surface protein labelling with nanometre precision, intracellular applications are limited by either the intrinsic toxicity of metal-based photocatalysts or by the lower resolution when long-lived reactive intermediates are used. Here we describe the discovery, characterization and application of an organic flavin cofactor derivative, deazaflavin, that activates diazirine to generate carbenes via triplet energy transfer and offers excellent biocompatibility. We demonstrate deazaflavin-diazirine energy-transfer labelling (DarT labelling) for cell surfaceome mapping and, most importantly,

SUBMITTER: Crocker LB 

PROVIDER: S-EPMC12669049 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature