Activation of the Dimer of 3, 4-dimethylphenol Production From Marine Streptomyces sp. FJNU027 Under Oligotrophic Condition.

ABSTRACT: Activating cryptic secondary metabolic gene clusters is a critical area of research in Streptomyces, and the cultivation-based approach is one of effective ways to induce the expression of cryptic gene clusters. In this study, the oligotrophic medium and modified Gauze's medium were used to culture the marine Streptomyces sp. FJNU027 strain, and a unique secondary metabolite in oligotrophic culture was found by HPLC assay when compared with the modified Gauze's culture. Then the differential product was isolated through large-scale fermentation, solvent extraction, column chromatography over Sephadex LH-20, and HPLC preparation. The pure differential product was analyzed by NMR and LC-MS, and identified as 4,4',5,5'-tetramethyl-[1,1'- diphenyl]-2,2'-diol. To elucidate the possible biosynth

SUBMITTER: Wang F 

PROVIDER: S-EPMC12669078 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature