Mendelian randomization integrated with multi-omics analysis identifies TNIK as a key gene in gut microbiota-induced IBD development.

ABSTRACT:

Background

Dysbiosis of the gut microbiota (GM) has been linked to inflammatory bowel disease (IBD), yet its associated molecular mechanisms remain poorly defined. Identifying causal host genes mediating GM-IBD interactions is therefore of great importance.

Objective

To identify GM-associated causal genes for IBD and to prioritize key targets and cell types underlying GM-host crosstalk.

Methods

We integrated GWAS datasets of GM, UC, and CD using a two-sample Mendelian randomization (MR) framework with IVW as the primary estimator. Causal SNPs were mapped to genes for enrichment analyses. Candidate genes were refined by intersecting MR-derived genes with bulk RNA-seq DEGs (training: GSE87473, validation: GSE75214) and prioritized using nested cross-validated machine-le

SUBMITTER: Chai X 

PROVIDER: S-EPMC12669205 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature