Metagenomic next-generation sequencing: a game-changer in the diagnosis of unique intraocular infections.

ABSTRACT:

Objective

To thoroughly describe unique intraocular infections diagnosed by metagenomic next-generation sequencing (mNGS).

Methods

A retrospective case series of patients presenting with challenging atypical intraocular infections at Tel Aviv Sourasky Medical Center during 2024. Clinical and demographic data, as well as mNGS results were extracted from patient records. mNGS was performed on the Illumina NextSeq500 platform using a custom bioinformatics pipeline. The following parameters were examined: Reads Per Million, Reads Per Million-ratio to negative control and E-index (K-mers*coverage/reads).

Results

The study included three patients with novel presentations of intraocular infections, manifesting with atypical clinical manifestations and negative routine diagno

SUBMITTER: Habot-Wilner Z 

PROVIDER: S-EPMC12669242 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature