An efficient electrotransformation method for three Bacillus species.

ABSTRACT: Bacillus strains are widely used in food fermentation and plant disease control. An efficient transformation method is crucial for genetic manipulation in these organisms. To enhance the transformation efficiency of three Bacillus strains-Bacillus amyloliquefaciens YN-J3 (B.a YN-J3), Bacillus velezensis JN-Y2 (B.v JN-Y2) and Bacillus subtilis S-16 (B.s S-16), we optimized transformation conditions using orthogonal experiments combined with response surface analysis. Additionally, we tested various cell wall agents to improve competence. Our results showed that the optimal transformation parameters for B.a YN-J3 and B.v JN-Y2 had an OD₆₀₀ of 0.70, a competent cell volume of 91 μL, a plasmid concentration of 1040 ng·μL⁻¹, and a field strength of 18.1 kV·cm⁻¹.

SUBMITTER: Quan W 

PROVIDER: S-EPMC12669251 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature