Functional landscape of ubiquitin linkages couples K29-linked ubiquitylation to epigenome integrity.

ABSTRACT: Linkage-specific ubiquitin chains govern the outcome of numerous critical ubiquitin-dependent signaling processes, but their targets and functional impacts remain incompletely understood due to a paucity of tools for their specific detection and manipulation. Here, we applied a cell-based ubiquitin replacement strategy enabling targeted conditional abrogation of each of the seven lysine-based ubiquitin linkages in human cells to profile system-wide impacts of disabling formation of individual chain types. This revealed proteins and processes regulated by each of these poly-ubiquitin topologies and indispensable roles of K48-, K63- and K27-linkages in cell proliferation. We show that K29-linked ubiquitylation is strongly associated with chromosome biology, and that the H3K9me3 methyltransfe

SUBMITTER: Arroyo-Gomez J 

PROVIDER: S-EPMC12669653 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature