Large-scale comparison of two immunoassays for adrenocorticotropic hormone in human plasma.

ABSTRACT: Accurate measurement of plasma adrenocorticotropic hormone (ACTH) is critical in the diagnosis and management of conditions that affect pituitary or adrenal gland function. Herein, we assessed the agreement and the extent of discordance between immunoassays from Roche and Siemens in two large collections of specimens. In a collection of 602 de-identified specimens (n = 561 from patients and n = 41 from healthy volunteers), the ACTH results were highly correlated with a Spearman correlation coefficient of 0.87. For all specimen comparisons, 56 (9.3%; 95% confidence interval [CI], 7.2%-11.9%) had a discordance with > 50% difference in the ACTH levels (including 2 healthy donors). In Bland-Altman analysis, 37 (6.1%; 95%CI, 4.5%-8.4%) specimens fell outside the limits of agreement (lower limit

SUBMITTER: Li Y 

PROVIDER: S-EPMC12669669 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature