Post-replicative initial expression of PAX6 during neuroectoderm differentiation.

ABSTRACT: The development of multicellular organisms requires precise coordination between cell division and differentiation. Cell division generates the necessary number of cells, while differentiation creates distinct cell identities, forming tissues and organs. The transcription factors SOX2 and PAX6 specify neuroepithelial cells, the earliest neural progenitor cells (NPCs) during brain development. How lineage specification is coordinated with the cell cycle is not fully understood. Here, we show that PAX6 expression occurs during a narrow time window after neural induction of human embryonic stem cells (ESCs). Flow cytometry analyses and time-lapse imaging show that PAX6 expression starts during the G2 phase. We identify a novel 500-bp PAX6 promoter that drives its G2-specific expression. PAX6

SUBMITTER: Hu S 

PROVIDER: S-EPMC12669799 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature