Marine Cold Seep ANME-2/SRB Consortia Produce Their Lipid Biomass From Inorganic Carbon.

ABSTRACT: In cold seeps, anaerobic methanotrophic archaea (ANME) and sulphate-reducing bacteria (SRB) oxidise methane to inorganic carbon (IC) coupled to sulphate reduction. While catabolic pathways are well resolved, carbon flow into biomass as well as the functional roles of lipid biomarkers remain unclear. We conducted lipid stable isotope probing (lipid-SIP) experiments with Astoria Canyon sediments dominated by ANME-2/SRB consortia and incubated samples with either ¹³C-labelled methane (¹³CH₄) or dissolved IC (DI¹³C). Lipid-specific δ¹³C analysis showed higher ¹³C incorporation from DI¹³C than from ¹³CH₄. After 30 days, δ¹³C values were up to +417‰ in SRB-specific fatty acids (e.g., C

SUBMITTER: Stock L 

PROVIDER: S-EPMC12669819 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature