Lamin B loss in nuclear blebs is rupture dependent whereas increased DNA damage is rupture independent.

ABSTRACT: The nucleus must maintain shape and integrity to protect the function of the genome. Nuclear blebs are deformations identified by decreased DNA density that commonly lead to rupture. Lamin B levels often vary drastically between blebs. We tracked rupture via time-lapse imaging of nuclear localization sequence (NLS)-GFP immediately followed by immunofluorescence imaging of lamins and known rupture markers. We find that lamin B1 loss consistently marks ruptured nuclear blebs better than lamin A/C, emerin and cGAS. Visualizing post-rupture lamin B1 loss and emerin enrichment reveals that cell lines display widely different propensities for nuclear bleb rupture. To determine how rupture affects DNA damage, we time-lapse-imaged ruptured and unruptured blebs, then conducted immunofluorescence on

SUBMITTER: Chu CG 

PROVIDER: S-EPMC12669966 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature