Neanderthal-derived variants increase SOX9 enhancer activity in craniofacial progenitors that shape jaw development.

ABSTRACT: Human facial appearance is a highly variable morphological trait, with both rare and common genetic variants shaping craniofacial morphology between individuals and in disease. Deletions encompassing an enhancer cluster 1.45 megabases upstream of SOX9 (EC1.45) cause Pierre Robin sequence, a craniofacial disorder characterised by underdevelopment of the lower jaw and associated with cleft palate. We hypothesised therefore that single nucleotide variants within EC1.45 may cause more subtle alterations in facial form. Leveraging recent human evolution, and the distinct jaw morphology of Neanderthals, we investigated the impact of three Neanderthal-derived variants on EC1.45 function. Using zebrafish dual enhancer-reporters, we observed higher activity of Neanderthal EC1.45 in neural crest-der

SUBMITTER: Uttley K 

PROVIDER: S-EPMC12669974 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature