Spatial transcriptomics mapping of immune cell and TGFβ signalling pathway heterogeneity in testicular germ cell tumours.

ABSTRACT:

Background

Testicular germ cell tumours (TGCTs) are amongst the most common malignancies in young men, and their incidence is increasing worldwide. Tissue heterogeneity hampers efforts to understand how TGCT precursors (termed germ cell neoplasia in situ; GCNIS) emerge and progress, restricting elucidation of new strategies for diagnosis and management.

Objectives

This study reports the use of spatial transcriptomic analysis in TGCT tissue sections.

Materials and methods

Over 90 regions of interest (ROIs) were identified in sections from four TGCT patients' samples, three with non-seminoma, one seminoma. Transcripts in each ROI were sequenced and examined using the NanoString GeoMx spatial whole transcriptomics workflow, the values normalised and analysed using Degust

SUBMITTER: Moody SC 

PROVIDER: S-EPMC12670472 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature