Meta-surface plasmon resonance microscopy for sensitive, high-throughput, and long-term label-free analysis of cell adhesion dynamics.

ABSTRACT: Quantitative, label-free monitoring of dynamic cell adhesion remains challenging. Meta-Surface Plasmon Resonance Microscopy (Meta-SPRM) is a novel platform integrating bright-field microscopy with engineered Meta-SPR nanocup arrays. This system simultaneously acquires bright-field images and Meta-SPRM signals, enabling their computational separation and co-analysis to provide multifaceted insights into cell-substrate interactions. Meta-SPRM offers sensitive, high-throughput, and long-term label-free quantification of cell adhesion strength and distribution. It captures dynamic processes like cell spreading and migration at micrometer lateral resolution. Notably, Meta-SPRM signals spatially correlate with key focal adhesion proteins (Integrin-β1, Vinculin), and an intrinsic intracellular si

SUBMITTER: Chen M 

PROVIDER: S-EPMC12670785 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature