GPX8 is transcriptionally regulated by KLF16 and promotes osteosarcoma progression.

ABSTRACT:

Background

Studies have shown that glutathione peroxidase 8 (GPX8) promotes the progression of various cancers. However, the role of GPX8 in osteosarcoma (OS) progression remains unclear. The aim of this study was to investigate the function of GPX8 in OS progression and its underlying mechanisms.

Methods

GPX8 expression in OS tissues and cell lines was assessed using immunohistochemistry (IHC), qRT‒PCR and Western blotting. The effects of GPX8 on the proliferative capacity of OS cells were evaluated using CCK-8, EdU, and plate colony formation assays. The role of GPX8 in the migratory and invasive functions of OS cells was examined via scratch wound healing, Transwell, and invasion assays. The effect of GPX8 on the in vivo proliferative ability of OS cells was investigated

SUBMITTER: Dong C 

PROVIDER: S-EPMC12670796 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature