Mega-scale single-cell profiling reveals novel biomarkers associated with acute GvHD after allogeneic hematopoietic stem cell transplantation.

ABSTRACT:

Background

Alloreactive T cells mediate graft-versus-leukemia (GvL) reactions and acute graft-versus-host disease (aGvHD) in AML patients following allogeneic hematopoietic stem cell transplantation.

Methods

To investigate biomarkers that identify alloreactive T cells associated with either beneficial GvL or detrimental aGvHD, we collected graft samples and two post-transplant follow-up blood samples (day 30 and day 100) of ten AML patients undergoing hematopoietic stem cell transplantation and profiled over 777,000 CD45⁺ leukocytes in total by combinatorial barcoding-based mega-scale single-cell RNA sequencing.

Results

Using immune receptor sequences as intrinsic clonal barcodes, we observed that especially CD8⁺ graft-derived T cells persisted a

SUBMITTER: Song Z 

PROVIDER: S-EPMC12670839 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature