Fuel-Free Rolosense: Viral Sensing Using Diffusional Particle Tracking.

ABSTRACT: High-sensitivity viral diagnostics typically use PCR to detect and amplify viral nucleic acids which requires fluorescence reporters, enzymatic amplification, specialized equipment and can be time-consuming. In this work, we describe fuel-free (FF) Rolosense, a diagnostic approach that leverages mechanical force sensing as a fundamental transduction mechanism. We use the Brownian motion of aptamer-coated microparticles on an aptamer-modified surface for viral detection. The microparticles function as both the sensing and transduction elements, reporting specific molecular interactions where the presence of viral particles stalls their motion by cross-linking them to the surface. FF-Rolosense harnesses biased motion and thermal fluctuations to achieve rapid, sensitive, and specific detectio

SUBMITTER: Piranej S 

PROVIDER: S-EPMC12670994 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature