Nanoengineered Calcium Receptors Coupled with Microscale Thermophoresis Enable Sensitive, Low-Volume Quantification of Calcium Ions in Complex Biological Fluids.

ABSTRACT: Accurate quantification of calcium ions (Ca²⁺) in biological fluids is essential for elucidating cellular physiology and diagnosing calcium-associated disorders, yet conventional analytical tools often face challenges, including limited sensitivity, interference from complex matrices, and the need for large sample volumes. Here, we report nanoengineered calcium receptors, Sens4Ca and Sens2Ca, integrated with microscale thermophoresis (MST) to achieve sensitive, robust Ca²⁺ detection using minimal sample volumes. The calcium receptors are modular fusion proteins that combine calmodulin and calmodulin-binding peptides with rigid structural domains to tailor binding affinities and stoichiometries, enabling Ca²⁺ detection across nanomolar to micromolar concentr

SUBMITTER: Franz P 

PROVIDER: S-EPMC12670996 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature