Production of glycolic acid through whole-cell bioconversion from PET monomer ethylene glycol using engineered Corynebacterium glutamicum.

ABSTRACT: In the last decade, the global warming and plastic pollution issue have driven research on developing a more sustainable platform for chemicals production from alternative feedstocks. Ethylene glycol (EG), a monomer of polyethylene terephthalate (PET) plastic, has a potential to become a renewable substrate for microbial production of value-added chemicals. This study presents a biotransformation platform using Corynebacterium glutamicum to produce glycolic acid (GA) from EG. C. glutamicum was engineered to express a heterologous EG oxidation pathway. Subsequent promoter engineering yielded strain FA4, producing 10.6 g/L GA from EG in 48 h. Implementation of a two-stage biotransformation strategy using resting cells further enhanced the GA production, reaching a cumulative GA titer of 98.8

SUBMITTER: Ghiffary MR 

PROVIDER: S-EPMC12671051 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature