Adaptive laboratory evolution of Saccharomyces cerevisiae CEN.PK 113-7D to enhance ethanol tolerance.

ABSTRACT: Saccharomyces cerevisiae is a widely used yeast for industrial production of ethanol. However, elevated ethanol, temperature, and osmotic stress adversely affect fermentation efficiency. In this study, adaptive laboratory evolution for S. cerevisiae CEN.PK 113-7D on higher concentrations of ethanol was performed. After 144 days, the maximum specific growth rate (µmax) increased from 0.0240 to 0.1150 h-1 for the strain evolved on 9% v/v ethanol, and from 0.0002 to 0.0530 h-1 for the strain evolved on 11% v/v ethanol, and the specific glucose uptake rate increased by 30%. The strain evolved on 11% ethanol produced 94.5 g/L ethanol in a fermentation as compared to 78.5 g/L production by a non-evolved strain. By whole-genome sequencing of the evolved clones, we identified multiple coding mutat

SUBMITTER: Sheikhi F 

PROVIDER: S-EPMC12671053 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature